Attaching Accessory Devices to the Replisome

Localization of an accessory helicase at the replisome is critical in sustaining efficient genome duplication

Genome duplication requires accessory helicases to displace proteins ahead of advancing replication forks. Escherichia coli contains three helicases, Rep, UvrD and DinG, that might promote replication of protein-bound DNA. One of these helicases, Rep, also interacts with the replicative helicase DnaB. We demonstrate that Rep is the only putative accessory helicase whose absence results in an in...

The accessory subunit B of DNA polymerase γ is required for mitochondrial replisome function

The mitochondrial replication machinery in human cells includes the DNA polymerase gamma holoenzyme and the TWINKLE helicase. Together, these two factors form a processive replication machinery, a replisome, which can use duplex DNA as template to synthesize long stretches of single-stranded DNA. We here address the importance of the smaller, accessory B subunit of DNA polymerase gamma and demo...

Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity

Replicative DNA helicases expose the two strands of the double helix to the replication apparatus, but accessory helicases are often needed to help forks move past naturally occurring hard-to-replicate sites, such as tightly bound proteins, RNA/DNA hybrids, and DNA secondary structures. Although the Schizosaccharomyces pombe 5'-to-3' DNA helicase Pfh1 is known to promote fork progression, its g...

SnapShot: The Replisome

Attaching handles to Bryant surfaces

Complete minimal surfaces and constant mean curvature surfaces in Euclidean or hyperbolic space have been the object of much attention in recent years and particular efforts have been devoted in understanding the moduli space of such surfaces. More precisely, the surfaces we are interested in are complete, noncompact surfaces which may or may not be embedded but enjoy the somehow weaker, and sl...